Purpose
1.To understand how the reversed—phase high performance liquid chromatography method to determine alkaloids and what the principle is.
2.To understand the characters of mobile phase which commonly used in reverse phase high performance liquid chromatography（RP—HPLC）and what should be to pay more attentions.
Principle
RP—HPLC is a type of reversed phase liquid chromatography which is defined as the polarity of the stationary phase is less than the mobile phase in a certain chromatographic system.The retention property of an analyte depends on it，s hydrophobicity that more strongly hydrophobic an analyte has，the more retentive the analyte is.Mobile phase in the modern HPLC generally is using so called"cherrucally bonded phase".For most of the mobile phase being used in the RP—HPLC，chemically bonded phase possesses good chemical stability，as well as the thermal stability.Except above，it also represents an advantage of high column efficiency，long column life，and good reproducibility.lt also provides possible to analyze more compounds that almost covers all types of organic compounds.
Conditions
1.Apparatus HPLC instrument，octadecyltrimethoxysilane column，microsyringe and developing chamber.
