Table of Contents

About the editors i
Preface iii
Acknowledgements v
Abbreviations vii
CHAPTER 1 Enzymes and their functions in soil and
groundwater 1
1.1 Introduction 1
1.2 Types and sources 3
1.2.1 Types 3
1.2.2 Sources 4
1.3 Kinetics， functions and influencing factors 5
1.3.1 Enzyme kinetics 5
1.3.2 Functions of enzymes 7
1.3.3 Influencing factors of enzyme activities 13
1.4 Methods for visualizing enzyme activities: in situ
zymography 21
1.4.1 Introduction 21
1.4.2 Materials and methods 24
1.4.3 Procedures 27
1.4.4 Combination with other visualization techniques 30
1.5 Enzymes and their relations with environmental researches 35
1.5.1 Effluent treatment and detoxification 36
1.5.2 Bioindicators for pollution monitoring 40
1.5.3 Biosensors 46
1.6 Conclusion 51
Main References 52
CHAPTER 2 Global climate change and enzyme activities 62
2.1 Global climate change and its mutual effects with the
environment 62
2.1.1 Overview of global climate change 62
2.1.2 Impacts of global climate change 63
2.1.3 Feedback of soil and groundwater environment to global
climate change 70
2.1.4 Summary and outlook 72
2.2 Temperature sensitivity of enzyme activities 74
2.2.1 Introduction 74
2.2.2 Temperature sensitivity of soil enzymes 76
2.2.3 Response of substrate affinity to temperature 79
2.2.4 Catalytic efficiency of enzymes as affected by temperature 82
2.2.5 Summary 84
Main References 84
CHAPTER 3 Response of enzyme activities to manure
applications 90
3.1 Impact of manure on soil biochemical properties: a global
synthesis 90
3.1.1 Introduction 90
3.1.2 Nutrient composition of manure and its effects on soil properties 92
3.1.3 Effects of manure application on enzyme activities 100
3.1.4 Soil pH and its influence on the manuring effect 109
3.1.5 Effects of soil， climate， management and manure-related factors
on the soil biochemical properties 111
3.1.6 Summary 114
3.2 Spatiotemporal patterns of enzyme activities after manure
application reflect mechanisms of niche differentiation
between plants and microorganisms 115
3.2.1 Introduction 115
3.2.2 Temporal response of enzyme activities to manure application
strategy 117
3.2.3 Spatial response of enzyme activities to manure application
strategies 124
3.2.4 Response of plants to manure application strategies 126
3.2.5 Summary 129
Main References 130
CHAPTER 4 Response of enzyme activities to metal/
nanometal oxide 140
4.1 Effects of nanometal oxides on enzyme activity 140
4.1.1 Introduction 140
4.1.2 Overview of nanometal oxides 141
4.1.3 Effect of ENOPs on enzyme activity 142
4.1.4 Major pathways to affect enzyme activities 142
4.1.5 Main regulators of enzyme activity 148
4.1.6 Summary and outlook 150
4.2 Effect of exogenous lead contamination on microbial
enzyme activity in purple soil 151
4.2.1 Introduction 151
4.2.2 Change in content of available lead 153
4.2.3 Sensitivity of enzyme activity to lead contamination 155
4.2.4 Dose-effect relationship between enzyme activity and lead
concentration 157
4.2.5 Summary 157
4.3 Toxicity of nano-CuO particles to maize and microbial
community largely depends on its bioavailable fractions 158
4.3.1 Introduction 158
4.3.2 Response of plants to nano-CuO and CuSO4 160
4.3.3 Response of microbial community compositions to nano-CuO
and CuSO4 164
4.3.4 Responses of enzyme activities on the rhizoplane to nano-CuO
and CuSO4 166
4.3.5 Agricultural implications 172
4.3.6 Summary 172
4.4 Influences of nano-ZnO particles on plant and microbes
grown in Pb-contaminated soil 172
4.4.1 Impact of ZnO nanoparticles on soil lead bioavailability and
microbial properties 172
4.4.2 Effects of nano ZnO addition on metal morphology and plant
growth in lead-contaminated soil 187
4.4.3 Summary 196
4.5 Effects of acid rain on heavy metal release and enzyme
activity in contaminated soil 198
4.5.1 Introduction 198
4.5.2 Effect of dynamic simulated acid rain on Cr(VI) release
characteristics and enzyme activity in contaminated soil 200
4.5.3 Effect of dynamic simulated acid rain on the release
characteristics and enzyme activity of Pb in contaminated soil 208
4.5.4 Summary 216
Main References 218
CHAPTER 5 Effect of biomass-based materials on
enzyme activities in heavy metal-
contaminated environment 229
5.1 Spatial-temporal distribution of enzyme activities in
heavy metal-contaminated soil after application
of organic fertilizers 229
5.1.1 Introduction 229
5.1.2 Impact of organic amendments on soil acidification 231
5.1.3 Impact of organic amendments on plant characteristics 234
5.1.4 Cr concentration and speciation in soil